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One particularly distinguishing feature in the spectroscopy of the eukaryotic SORs studied to date is the appearance of = 1/2 superhyperfine splitting of the Mo(V) EPR signal at low p H.

Gels were stained for protein using either Coomassie Brilliant Blue or silver (24).However, more extensive characterization of the type of pterin molybdenum cofactor contained in the enzyme, the exact nature of the heme group, and further properties of this or any other of the (16)), which encodes an enzyme that is part of a multienzyme complex.The present work describes the first spectroscopic and enzymological characterization of a bacterial member of the sulfite oxidase family, the SOR from and thiosulfate were autoclaved separately. 200-ml cultures were aerated by vigorous shaking and incubated for 3–4 days at 30 °C; 10-liter cultures were grown in carboys with forced aeration over 6–7 days. After 2–3 days, 500 ml of a thiosulfate feeding solution (200 g/l) were added to 10-liter cultures every second day.Extinction coefficients for cytochrome under partly inhibiting conditions as described in Ref.19 to overcome detection limits of measurements with a light path of 1 cm.




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